From: Subject: Concise Summary Report (PRL-S1) Date: Wed, 5 Mar 2008 12:39:45 -0500 MIME-Version: 1.0 Content-Type: multipart/related; type="text/html"; boundary="----=_NextPart_000_0000_01C87EBD.FDBC6480" X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 This is a multi-part message in MIME format. ------=_NextPart_000_0000_01C87EBD.FDBC6480 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Content-Location: http://localhost/mascot/cgi/master_results.pl?file=../data/20080305/F002800.dat Concise Summary Report (PRL-S1)

= Mascot Search=20 Results

User    =
        : Protea PRL
Email           : 
Search title    : PRL-S1
Database        : UniProtKB-SwissProt sprot_20070123 (254609 =
sequences; 93000223 residues)
Taxonomy        : Mammalia (mammals) (49274 sequences)
Timestamp       : 5 Mar 2008 at 17:37:44 GMT
Warning         : Specifying a protein mass disables mixture mode in =
PMF-search
Top Score       : 103 for P02769, Serum =
albumin precursor (Allergen Bos d 6) (BSA) - Bos taurus (Bovine)

Probability Based Mowse Score

Ions score is -10*Log(P), where P = is the=20 probability that the observed match is a random event.
Protein scores = greater=20 than 59 are significant (p<0.05).

3D"Score=20

=20

Concise Protein Summary Report

Help
  Significance threshold p< Max. number of hits
= =20 =20 =20 = =20 =20 =20 =20 =20 =20 = =20 =20 =20 =20 = =20 =20 = =20 =20 =20 =20 = =20 =20 = =20 =20 = =20 = =20 = = = = =20

  =20

1.     P02769        = ;            =         Mass: 71244&n= bsp;   Score: 103    Expect:&n= bsp;2.5e-006  Queries=20 matched: 27
  Serum albumin precursor (Allergen Bos d 6) (BSA) - = Bos=20 taurus (Bovine)

2.     Q6NZL0        = ;            =         Mass: 103758&= nbsp;  Score: 44     Ex= pect: 2  Queries=20 matched: 19
  Uncharacterized protein C6orf174 homolog precursor - = Mus=20 musculus (Mouse)
3.     Q5RB75        = ;            =         Mass: 40782&n= bsp;   Score: 37     = Expect: 9.8  Queries=20 matched: 14
  NudC domain-containing protein 3 - Pongo pygmaeus=20 (Orangutan)
4.     Q5T6X5        = ;            =         Mass: 106623&= nbsp;  Score: 37     Ex= pect: 11  Queries=20 matched: 16
  G-protein coupled receptor family C group 6 member A=20 precursor (hGPRC6A) (G-protein coupled = recepto
5.     Q5TF21        = ;            =         Mass: 103478&= nbsp;  Score: 35     Ex= pect: 17  Queries=20 matched: 16
  Uncharacterized protein C6orf174 precursor - Homo = sapiens=20 (Human)
6.     Q9CWX2        = ;            =         Mass: 37900&n= bsp;   Score: 33     = Expect: 28  Queries=20 matched: 12
  Complex I intermediate-associated protein 30, = mitochondrial=20 precursor (NADH dehydrogenase = [ubiquino
7.     P14639        = ;            =         Mass: 71139&n= bsp;   Score: 32     = Expect: 29  Queries=20 matched: 16
  Serum albumin precursor - Ovis aries=20 (Sheep)
8.     Q9Y5W8        = ;            =         Mass: 112744&= nbsp;  Score: 31     Ex= pect: 37  Queries=20 matched: 16
  Sorting nexin-13 (RGS domain- and PHOX = domain-containing=20 protein) (RGS-PX1) - Homo sapiens = (Human)
9.     Q8K4E0        = ;            =         Mass: 362981&= nbsp;  Score: 31     Ex= pect: 43  Queries=20 matched: 14
  Alstrom syndrome protein 1 homolog - Mus musculus=20 (Mouse)
10.    P01570        = ;            =         Mass: 22390&n= bsp;   Score: 30     = Expect: 48  Queries=20 matched: 9
  Interferon alpha-14 precursor (Interferon alpha-H) = (LeIF H)=20 (Interferon lambda-2-H) - Homo sapiens =
11.    P20156        = ;            =         Mass: 68209&n= bsp;   Score: 30     = Expect: 53  Queries=20 matched: 14
  Neurosecretory protein VGF precursor (VGF8a protein) = -=20 Rattus norvegicus (Rat)
12.    Q717B4        = ;            =         Mass: 42226&n= bsp;   Score: 29     = Expect: 61  Queries=20 matched: 11
  TD and POZ domain-containing protein 3 - Mus musculus = (Mouse)
13.    Q9JKD9        = ;            =         Mass: 51537&n= bsp;   Score: 29     = Expect: 65  Queries=20 matched: 13
  Zinc finger and BTB domain-containing protein 32 = (Repressor=20 of GATA) (Testis zinc finger protein) = -
14.    P32195        = ;            =         Mass: 16981&n= bsp;   Score: 29     = Expect: 65  Queries=20 matched: 7
  Protegrin-2 precursor (PG-2) - Sus scrofa=20 (Pig)
       P32194        = ;            =         Mass: 17180&n= bsp;   Score: 28     = Expect: 71  Queries=20 matched: 7
  Protegrin-1 precursor (PG-1) (Neutrophil peptide 1) - = Sus=20 scrofa (Pig)
15.    Q8R071        = ;            =         Mass: 51359&n= bsp;   Score: 29     = Expect: 68  Queries=20 matched: 13
  Inositol-trisphosphate 3-kinase A (EC 2.7.1.127) = (Inositol=20 1,4,5-trisphosphate 3-kinase A) (IP3K = A)
16.    Q5E9K0        = ;            =         Mass: 22996&n= bsp;   Score: 29     = Expect: 68  Queries=20 matched: 8
  Proteasome subunit beta type 2 (EC 3.4.25.1) - Bos = taurus=20 (Bovine)
17.    Q811C2        = ;            =         Mass: 52680&n= bsp;   Score: 29     = Expect: 68  Queries=20 matched: 12
  Cysteine protease ATG4C (EC 3.4.22.-) = (Autophagy-related=20 protein 4 homolog C) (Autophagin-3) = (Autop
18.    P00126        = ;            =         Mass: 10902&n= bsp;   Score: 28     = Expect: 73  Queries=20 matched: 6
  Ubiquinol-cytochrome c reductase complex 11 kDa = protein,=20 mitochondrial precursor (EC 1.10.2.2) = (Mit
19.    P02770        = ;            =         Mass: 70682&n= bsp;   Score: 28     = Expect: 75  Queries=20 matched: 15
  Serum albumin precursor - Rattus norvegicus=20 (Rat)
20.    P46100        = ;            =         Mass: 284843&= nbsp;  Score: 28     Ex= pect: 86  Queries=20 matched: 16
  Transcriptional regulator ATRX (EC 3.6.1.-) = (ATP-dependent=20 helicase ATRX) (X-linked helicase II) = (X

Search Parameters

Type of search         : Peptide Mass =
Fingerprint
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Variable modifications : Oxidation (M)
Mass values            : Monoisotopic
Protein Mass           : 66 kDa
Peptide Mass Tolerance : =B1 1 Da
Peptide Charge State   : 1+
Max Missed Cleavages   : 2
Number of queries      : 50

Mascot:  http://www.matrixscience.com/ =

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Content-Transfer-Encoding: quoted-printable Content-Location: http://localhost/mascot/templates/browser.js var browser_EXCLUDE =3D 1;=20 var browser_IE =3D 0;=20 var browser_IE4 =3D 0;=20 var browser_IE5 =3D 0;=20 var browser_IE6 =3D 0; var browser_MZ7 =3D 0; var browser_NS3 =3D 0; var browser_NS4 =3D 0; var browser_NS6 =3D 0;=20 var browser_NS7 =3D 0;=20 var browser_OP5 =3D 0;=20 var browser_OP6 =3D 0; var browser_OP7 =3D 0; var browser_KDE =3D 0; var browser_SAF =3D 0; var agt=3Dnavigator.userAgent.toLowerCase(); if(typeof navigator.vendor !=3D "undefined" && navigator.vendor =3D=3D = "KDE"){ var thisKDE =3D agt; var splitKDE =3D thisKDE.split("konqueror/"); var aKDE =3D splitKDE[1] . split("; "); var KDEn =3D parseFloat(aKDE[0]); if (KDEn >=3D 2.2) { browser_KDE =3D 1; // browser_NS6 =3D 1; browser_EXCLUDE =3D 0; } } else if(agt.indexOf('webtv') !=3D -1){browser_EXCLUDE =3D 1;} =20 else if(typeof window.opera !=3D "undefined"){ browser_EXCLUDE =3D 0; if(agt.indexOf("opera/5") !=3D -1 || agt.indexOf("opera 5") !=3D = -1){browser_OP5 =3D 1;} if(agt.indexOf("opera/6") !=3D -1 || agt.indexOf("opera 6") !=3D = -1){browser_OP6 =3D 1;} if(agt.indexOf("opera/7") !=3D -1 || agt.indexOf("opera 7") !=3D = -1){browser_OP7 =3D 1;} } =20 else if(typeof document.all !=3D "undefined"){ browser_EXCLUDE =3D 0; browser_IE =3D 1; if(typeof document.getElementById !=3D "undefined"){ browser_IE5 =3D 1; if(agt.indexOf("msie 6") !=3D -1){ browser_IE6 =3D 1; } } else{browser_IE4 =3D 1;} } =20 else if(typeof document.getElementById !=3D "undefined"){ browser_EXCLUDE =3D 0; if(agt.indexOf("netscape/6") !=3D -1 || agt.indexOf("netscape6") !=3D = -1){browser_NS6 =3D 1;} else if(agt.indexOf("netscape/7") !=3D -1 || agt.indexOf("netscape7") = !=3D -1){browser_NS6 =3D 1; browser_NS7 =3D 1;} else if(agt.indexOf("gecko") !=3D -1){browser_NS6 =3D 1; browser_MZ7 = =3D 1;} if(agt.indexOf("safari") !=3D -1 || (typeof document.childNodes !=3D = "undefined" && typeof document.all =3D=3D "undefined" && typeof = navigator.taintEnabled =3D=3D "undefined")){browser_MZ7 =3D 0; = browser_NS6 =3D 1; browser_SAF =3D 1;} } =20 else if((agt.indexOf('mozilla') !=3D -1) && = (parseInt(navigator.appVersion) >=3D 3)){ browser_EXCLUDE =3D 0; if (parseInt(navigator.appVersion) >=3D 4) { browser_NS4 =3D 1; } else { browser_NS3 =3D 1; } if(typeof navigator.mimeTypes['*'] =3D=3D "undefined"){ browser_EXCLUDE =3D 1; browser_NS4 =3D 0; browser_NS3 =3D 0; } } =20 if(agt.indexOf('escape') !=3D -1){browser_EXCLUDE =3D 1; browser_NS3 = =3D 0; browser_NS4 =3D 0;} =20 if(typeof navigator.__ice_version !=3D "undefined"){browser_EXCLUDE = =3D 1; browser_IE4 =3D 0;} var sTemp =3D "Browser detected as "; if (browser_EXCLUDE) { sTemp +=3D "EXCLUDE " } if (browser_IE) { sTemp +=3D "IE " } if (browser_IE4) { sTemp +=3D "IE4 " } if (browser_IE5) { sTemp +=3D "IE5 " } if (browser_IE6) { sTemp +=3D "IE6 " } if (browser_MZ7) { sTemp +=3D "MZ7 " } if (browser_NS3) { sTemp +=3D "NS3 " } if (browser_NS4) { sTemp +=3D "NS4 " } if (browser_NS6) { sTemp +=3D "NS6 " } if (browser_NS7) { sTemp +=3D "NS7 " } if (browser_OP5) { sTemp +=3D "OP5 " } if (browser_OP6) { sTemp +=3D "OP6 " } if (browser_OP7) { sTemp +=3D "OP7 " } if (browser_KDE) { sTemp +=3D "KDE " } if (browser_SAF) { sTemp +=3D "SAF " } // alert(sTemp); ------=_NextPart_000_0000_01C87EBD.FDBC6480--