From: Subject: Concise Summary Report (PRL-S2) Date: Wed, 5 Mar 2008 12:44:29 -0500 MIME-Version: 1.0 Content-Type: multipart/related; type="text/html"; boundary="----=_NextPart_000_0009_01C87EBE.A78B4F30" X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 This is a multi-part message in MIME format. ------=_NextPart_000_0009_01C87EBE.A78B4F30 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Content-Location: http://localhost/mascot/cgi/master_results.pl?file=../data/20080305/F002806.dat Concise Summary Report (PRL-S2)

= Mascot Search=20 Results

User    =
        : Protea PRL
Email           : 
Search title    : PRL-S2
Database        : UniProtKB-SwissProt sprot_20070123 (254609 =
sequences; 93000223 residues)
Timestamp       : 5 Mar 2008 at 17:43:43 GMT
Warning         : Specifying a protein mass disables mixture mode in =
PMF-search
Top Score       : 60 for P01012, =
Ovalbumin (Plakalbumin) (Allergen Gal d 2) (Gal d II) - Gallus gallus =
(Chicken)

Probability Based Mowse Score

Ions score is -10*Log(P), where P = is the=20 probability that the observed match is a random event.
Protein scores = greater=20 than 67 are significant (p<0.05).

3D"Score=20

=20

Concise Protein Summary Report

Help
  Significance threshold p< Max. number of hits
= =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 =20 =20 =20 = =20 =20 =20 =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 = =20 = = =20

  =20

1.     P01012        = ;            =         Mass: 43196&n= bsp;   Score: 60     = Expect: 0.26  Queries=20 matched: 15
  Ovalbumin (Plakalbumin) (Allergen Gal d 2) (Gal d II) = -=20 Gallus gallus (Chicken)

2.     Q57L31        = ;            =         Mass: 28460&n= bsp;   Score: 49     = Expect: 3.3  Queries=20 matched: 10
  tRNA (guanine-N(1)-)-methyltransferase (EC 2.1.1.31)=20 (M1G-methyltransferase) (tRNA [GM37] = methyltra
       Q5PFF8        = ;            =         Mass: 28415&n= bsp;   Score: 47     = Expect: 5.2  Queries=20 matched: 10
  tRNA (guanine-N(1)-)-methyltransferase (EC 2.1.1.31)=20 (M1G-methyltransferase) (tRNA [GM37] = methyltra
       Q8Z4I5        = ;            =         Mass: 28415&n= bsp;   Score: 47     = Expect: 5.2  Queries=20 matched: 10
  tRNA (guanine-N(1)-)-methyltransferase (EC 2.1.1.31)=20 (M1G-methyltransferase) (tRNA [GM37] = methyltra
       P36245        = ;            =         Mass: 28445&n= bsp;   Score: 47     = Expect: 5.2  Queries=20 matched: 10
  tRNA (guanine-N(1)-)-methyltransferase (EC 2.1.1.31)=20 (M1G-methyltransferase) (tRNA [GM37] = methyltra
3.     P16785        = ;            =         Mass: 254719&= nbsp;  Score: 45     Ex= pect: 8.6  Queries=20 matched: 10
  Probable large tegument protein - Human = cytomegalovirus=20 (strain AD169) (HHV-5) (Human herpesvirus = 5
4.     Q87X13        = ;            =         Mass: 10261&n= bsp;   Score: 44     = Expect: 11  Queries=20 matched: 6
  10 kDa chaperonin (Protein Cpn10) (groES protein) -=20 Pseudomonas syringae pv. tomato
5.     P20796        = ;            =         Mass: 159553&= nbsp;  Score: 42     Ex= pect: 15  Queries=20 matched: 10
  Adhesin P1 precursor (Cytadhesin P1) (Attachment = protein)=20 (MgPa) - Mycoplasma genitalium
6.     Q8VSR0        = ;            =         Mass: 67098&n= bsp;   Score: 42     = Expect: 18  Queries=20 matched: 10
  Sulfite reductase [NADPH] hemoprotein beta-component = (EC=20 1.8.1.2) (SIR-HP) (SIRHP) - = Actinobacillus
7.     O75426        = ;            =         Mass: 66158&n= bsp;   Score: 41     = Expect: 20  Queries=20 matched: 10
  F-box only protein 24 - Homo sapiens=20 (Human)
       Q4R327        = ;            =         Mass: 66132&n= bsp;   Score: 41     = Expect: 20  Queries=20 matched: 10
  F-box only protein 24 - Macaca fascicularis (Crab = eating=20 macaque) (Cynomolgus monkey)
8.     P37024        = ;            =         Mass: 89491&n= bsp;   Score: 41     = Expect: 21  Queries=20 matched: 11
  ATP-dependent RNA helicase hrpB (EC 3.6.1.-) - = Escherichia=20 coli
9.     Q6ZMW2        = ;            =         Mass: 82734&n= bsp;   Score: 41     = Expect: 22  Queries=20 matched: 11
  Zinc finger protein 782 - Homo sapiens=20 (Human)
10.    Q8CCN1        = ;            =         Mass: 77288&n= bsp;   Score: 40     = Expect: 27  Queries=20 matched: 11
  NACHT, LRR and PYD-containing protein 10 - Mus = musculus=20 (Mouse)
11.    Q68X45        = ;            =         Mass: 89540&n= bsp;   Score: 40     = Expect: 28  Queries=20 matched: 9
  DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase = I)=20 (Omega-protein) (Relaxing enzyme) = (Untwisti
12.    Q9D417        = ;            =         Mass: 66756&n= bsp;   Score: 40     = Expect: 29  Queries=20 matched: 10
  F-box only protein 24 - Mus musculus=20 (Mouse)
13.    P11277        = ;            =         Mass: 247025&= nbsp;  Score: 39     Ex= pect: 30  Queries=20 matched: 11
  Spectrin beta chain, erythrocyte (Beta-I spectrin) - = Homo=20 sapiens (Human)
14.    Q53727        = ;            =         Mass: 84249&n= bsp;   Score: 39     = Expect: 31  Queries=20 matched: 11
  ATP-dependent DNA helicase pcrA (EC 3.6.1.-) -=20 Staphylococcus aureus (strain NCTC = 8325)
       Q5HEL7        = ;            =         Mass: 84249&n= bsp;   Score: 39     = Expect: 31  Queries=20 matched: 11
  ATP-dependent DNA helicase pcrA (EC 3.6.1.-) -=20 Staphylococcus aureus (strain COL)
       Q6G828        = ;            =         Mass: 84249&n= bsp;   Score: 39     = Expect: 31  Queries=20 matched: 11
  ATP-dependent DNA helicase pcrA (EC 3.6.1.-) -=20 Staphylococcus aureus (strain = MSSA476)
       Q8NVT1        = ;            =         Mass: 84249&n= bsp;   Score: 39     = Expect: 31  Queries=20 matched: 11
  ATP-dependent DNA helicase pcrA (EC 3.6.1.-) -=20 Staphylococcus aureus (strain MW2)
15.    Q6C3X7        = ;            =         Mass: 54640&n= bsp;   Score: 39     = Expect: 31  Queries=20 matched: 10
  ATP-dependent RNA helicase DBP5 (EC 3.6.1.-) - = Yarrowia=20 lipolytica (Candida lipolytica)
16.    Q9L0L6        = ;            =         Mass: 37878&n= bsp;   Score: 39     = Expect: 33  Queries=20 matched: 8
  Probable adenosine deaminase 4 (EC 3.5.4.4) = (Adenosine=20 aminohydrolase 4) - Streptomyces = coelicolor
17.    P22307        = ;            =         Mass: 59640&n= bsp;   Score: 39     = Expect: 35  Queries=20 matched: 11
  Nonspecific lipid-transfer protein (EC 2.3.1.176)=20 (Propanoyl-CoA C-acyltransferase) (NSL-TP) = (Stero
18.    Q4I9B7        = ;            =         Mass: 125250&= nbsp;  Score: 38     Ex= pect: 39  Queries=20 matched: 10
  Protein STU1 - Gibberella zeae (Fusarium=20 graminearum)
19.    Q5QV03        = ;            =         Mass: 21771&n= bsp;   Score: 38     = Expect: 40  Queries=20 matched: 7
  Peptidyl-tRNA hydrolase (EC 3.1.1.29) (PTH) - = Idiomarina=20 loihiensis
20.    P49792        = ;            =         Mass: 362365&= nbsp;  Score: 38     Ex= pect: 40  Queries=20 matched: 10
  E3 SUMO-protein ligase RanBP2 (Ran-binding protein 2) = (Nuclear pore complex protein Nup358) = (Nucleo

Search Parameters

Type of search         : Peptide Mass =
Fingerprint
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Variable modifications : Oxidation (M)
Mass values            : Monoisotopic
Protein Mass           : 45 kDa
Peptide Mass Tolerance : =B1 0.6 Da
Peptide Charge State   : 1+
Max Missed Cleavages   : 2
Number of queries      : 44

Mascot:  http://www.matrixscience.com/ =

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