From: Subject: Concise Summary Report (PRL-S3) Date: Wed, 5 Mar 2008 12:54:07 -0500 MIME-Version: 1.0 Content-Type: multipart/related; type="text/html"; boundary="----=_NextPart_000_0012_01C87EBF.FFA62130" X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 This is a multi-part message in MIME format. ------=_NextPart_000_0012_01C87EBF.FFA62130 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Content-Location: http://localhost/mascot/cgi/master_results.pl?file=../data/20080305/F002820.dat Concise Summary Report (PRL-S3)

= Mascot Search=20 Results

User    =
        : Protea PRL
Email           : 
Search title    : PRL-S3
Database        : UniProtKB-SwissProt sprot_20070123 (254609 =
sequences; 93000223 residues)
Taxonomy        : Mammalia (mammals) (49274 sequences)
Timestamp       : 5 Mar 2008 at 17:53:35 GMT
Top Score       : 50 for P00004, =
Cytochrome c - Equus caballus (Horse)

Probability Based Mowse Score

Ions score is -10*Log(P), where P = is the=20 probability that the observed match is a random event.
Protein scores = greater=20 than 59 are significant (p<0.05).

3D"Score=20

=20

Concise Protein Summary Report

Help
  Significance threshold p< Max. number of hits
= =20 =20 = =20 =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 =20 =20 = =20 =20 =20 = =20 =20 =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 = =20 = =20 = = =20 = = = = =20

  =20

1.     P00004        = ;            =         Mass: 11939&n= bsp;   Score: 50     = Expect: 0.49  Queries=20 matched: 17
  Cytochrome c - Equus caballus=20 (Horse)
       P68097        = ;            =         Mass: 11925&n= bsp;   Score: 37     = Expect: 11  Queries=20 matched: 15
  Cytochrome c - Equus asinus=20 (Donkey)
       P68096        = ;            =         Mass: 11925&n= bsp;   Score: 37     = Expect: 11  Queries=20 matched: 15
  Cytochrome c - Equus burchelli (Plains zebra) (Equus=20 quagga)

2.     Q6PCT3        = ;            =         Mass: 24034&n= bsp;   Score: 40     = Expect: 4.6  Queries=20 matched: 20
  Tumor protein D54 (Tumor protein D52-like 2) - Rattus = norvegicus (Rat)
       Q9CYZ2        = ;            =         Mass: 24085&n= bsp;   Score: 35     = Expect: 17  Queries=20 matched: 19
  Tumor protein D54 (Tumor protein D52-like 2) - Mus = musculus=20 (Mouse)
3.     Q9D924        = ;            =         Mass: 14351&n= bsp;   Score: 40     = Expect: 4.7  Queries=20 matched: 13
  HESB-like domain-containing protein 2, mitochondrial=20 precursor (Iron sulfur assembly protein IscA) =
4.     Q27996        = ;            =         Mass: 16374&n= bsp;   Score: 39     = Expect: 5.9  Queries=20 matched: 13
  Lysozyme C, tracheal isozyme precursor (EC 3.2.1.17)=20 (1,4-beta-N-acetylmuramidase C) - Bos taurus = (
5.     Q5R7E0        = ;            =         Mass: 25119&n= bsp;   Score: 38     = Expect: 8.4  Queries=20 matched: 15
  Peroxiredoxin-6 (EC 1.11.1.15) - Pongo pygmaeus=20 (Orangutan)
6.     Q8N283        = ;            =         Mass: 110811&= nbsp;  Score: 37     Ex= pect: 10  Queries=20 matched: 38
  Ankyrin repeat domain-containing protein 35 - Homo = sapiens=20 (Human)
7.     Q9NZ50        = ;            =         Mass: 13245&n= bsp;   Score: 37     = Expect: 10  Queries=20 matched: 11
  Gamma-synuclein (Synoretin) (SR) - Bos taurus=20 (Bovine)
8.     Q61598        = ;            =         Mass: 51018&n= bsp;   Score: 36     = Expect: 12  Queries=20 matched: 25
  Rab GDP dissociation inhibitor beta (Rab GDI beta)=20 (Guanosine diphosphate dissociation inhibitor = 2)
       P50399        = ;            =         Mass: 51018&n= bsp;   Score: 36     = Expect: 12  Queries=20 matched: 25
  Rab GDP dissociation inhibitor beta (Rab GDI beta)=20 (Guanosine diphosphate dissociation inhibitor = 2)
9.     P54921        = ;            =         Mass: 33627&n= bsp;   Score: 34     = Expect: 18  Queries=20 matched: 18
  Alpha-soluble NSF attachment protein (SNAP-alpha)=20 (N-ethylmaleimide-sensitive factor attachment = pro
10.    P30041        = ;            =         Mass: 25133&n= bsp;   Score: 34     = Expect: 20  Queries=20 matched: 15
  Peroxiredoxin-6 (EC 1.11.1.15) (Antioxidant protein = 2)=20 (1-Cys peroxiredoxin) (1-Cys PRX) (Acidic = ca
11.    Q3T9Z9        = ;            =         Mass: 66863&n= bsp;   Score: 33     = Expect: 25  Queries=20 matched: 30
  Zinc finger protein C6orf113 homolog - Mus musculus=20 (Mouse)
12.    P27124        = ;            =         Mass: 51785&n= bsp;   Score: 32     = Expect: 34  Queries=20 matched: 25
  FK506-binding protein 4 (EC 5.2.1.8) (Peptidyl-prolyl = cis-trans isomerase) (PPIase) (Rotamase) = (p59
13.    P69341        = ;            =         Mass: 6531&nb= sp;    Score: 31    &= nbsp;Expect: 36  Queries=20 matched: 9
  Poly(A)-specific ribonuclease PARN (EC 3.1.13.4)=20 (Polyadenylate-specific ribonuclease)=20 (Deadenylati
14.    P48036        = ;            =         Mass: 35787&n= bsp;   Score: 31     = Expect: 43  Queries=20 matched: 16
  Annexin A5 (Annexin V) (Lipocortin V) (Endonexin II)=20 (Calphobindin I) (CBP-I) (Placental = anticoagul
15.    P68083        = ;            =         Mass: 17072&n= bsp;   Score: 30     = Expect: 48  Queries=20 matched: 13
  Myoglobin - Equus burchelli (Plains zebra) (Equus=20 quagga)
       P68082        = ;            =         Mass: 17072&n= bsp;   Score: 30     = Expect: 48  Queries=20 matched: 13
  Myoglobin - Equus caballus = (Horse)
16.    Q62826        = ;            =         Mass: 74076&n= bsp;   Score: 30     = Expect: 48  Queries=20 matched: 39
  Heterogeneous nuclear ribonucleoprotein M (hnRNP M) = (M4=20 protein) - Rattus norvegicus (Rat)
17.    O46563        = ;            =         Mass: 56272&n= bsp;   Score: 30     = Expect: 48  Queries=20 matched: 27
  Vacuolar ATP synthase subunit H (EC 3.6.3.14) = (V-ATPase H=20 subunit) (Vacuolar proton pump subunit = H)
18.    Q9DB05        = ;            =         Mass: 33624&n= bsp;   Score: 30     = Expect: 49  Queries=20 matched: 17
  Alpha-soluble NSF attachment protein (SNAP-alpha)=20 (N-ethylmaleimide-sensitive factor attachment = pro
19.    P07320        = ;            =         Mass: 21067&n= bsp;   Score: 30     = Expect: 50  Queries=20 matched: 13
  Gamma crystallin D (Gamma crystallin 4) - Homo = sapiens=20 (Human)
20.    O70183        = ;            =         Mass: 28689&n= bsp;   Score: 29     = Expect: 58  Queries=20 matched: 15
  Brain-derived neurotrophic factor precursor (BDNF) - = Cavia=20 porcellus (Guinea pig)

Search Parameters

Type of search         : Peptide Mass =
Fingerprint
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Variable modifications : Oxidation (M)
Mass values            : Monoisotopic
Protein Mass           : Unrestricted
Peptide Mass Tolerance : =B1 1 Da
Peptide Charge State   : 1+
Max Missed Cleavages   : 2
Number of queries      : 102

Mascot:  http://www.matrixscience.com/ =

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detected as "; if (browser_EXCLUDE) { sTemp +=3D "EXCLUDE " } if (browser_IE) { sTemp +=3D "IE " } if (browser_IE4) { sTemp +=3D "IE4 " } if (browser_IE5) { sTemp +=3D "IE5 " } if (browser_IE6) { sTemp +=3D "IE6 " } if (browser_MZ7) { sTemp +=3D "MZ7 " } if (browser_NS3) { sTemp +=3D "NS3 " } if (browser_NS4) { sTemp +=3D "NS4 " } if (browser_NS6) { sTemp +=3D "NS6 " } if (browser_NS7) { sTemp +=3D "NS7 " } if (browser_OP5) { sTemp +=3D "OP5 " } if (browser_OP6) { sTemp +=3D "OP6 " } if (browser_OP7) { sTemp +=3D "OP7 " } if (browser_KDE) { sTemp +=3D "KDE " } if (browser_SAF) { sTemp +=3D "SAF " } // alert(sTemp); ------=_NextPart_000_0012_01C87EBF.FFA62130--