From: Subject: Concise Summary Report (PRL-S4) Date: Wed, 5 Mar 2008 13:16:11 -0500 MIME-Version: 1.0 Content-Type: multipart/related; type="text/html"; boundary="----=_NextPart_000_001B_01C87EC3.15349830" X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 This is a multi-part message in MIME format. ------=_NextPart_000_001B_01C87EC3.15349830 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Content-Location: http://localhost/mascot/cgi/master_results.pl?file=../data/20080305/F002830.dat Concise Summary Report (PRL-S4)

= Mascot Search=20 Results

User    =
        : Protea PRL
Email           : 
Search title    : PRL-S4
Database        : UniProtKB-SwissProt sprot_20070123 (254609 =
sequences; 93000223 residues)
Taxonomy        : Mammalia (mammals) (49274 sequences)
Timestamp       : 5 Mar 2008 at 18:15:40 GMT
Warning         : Specifying a protein mass disables mixture mode in =
PMF-search
Top Score       : 163 for P68083, =
Myoglobin - Equus burchelli (Plains zebra) (Equus quagga)

Probability Based Mowse Score

Ions score is -10*Log(P), where P = is the=20 probability that the observed match is a random event.
Protein scores = greater=20 than 59 are significant (p<0.05).

3D"Score =20

= =20 =20 = =20 =20 =20 =20 = =20 =20 = =20 =20 =20 = =20 =20 = =20 =20 = =20 = =20 = = = = =20

=20

1.     P68083        = ;            =         Mass: 17072&n= bsp;   Score: 163    Expect:&n= bsp;2.5e-012  Queries=20 matched: 20

Myoglobin - Equus burchelli (Plains zebra) (Equus=20 quagga)

       P68082        = ;            =         Mass: 17072&n= bsp;   Score: 163    Expect:&n= bsp;2.5e-012  Queries=20 matched: 20

Myoglobin - Equus caballus = (Horse)

       P02166        = ;            =         Mass: 17052&n= bsp;   Score: 76     Expect:<= /B> 0.0014  Queries=20 matched: 12

Myoglobin - Perodicticus potto edwarsi=20 (Potto)

       P02168        = ;            =         Mass: 17091&n= bsp;   Score: 54     = Expect: 0.2  Queries=20 matched: 10

Myoglobin - Galago crassicaudatus (Thick-tailed = galago)=20 (Otolemur crassicaudatus)

       P02186        = ;            =         Mass: 17116&n= bsp;   Score: 47     = Expect: 0.9  Queries=20 matched: 10

Myoglobin - Elephas maximus (Indian=20 elephant)

       P02187        = ;            =         Mass: 17142&n= bsp;   Score: 47     = Expect: 0.9  Queries=20 matched: 10

Myoglobin - Loxodonta africana (African=20 elephant)

       P02167        = ;            =         Mass: 16944&n= bsp;   Score: 43     = Expect: 2.4  Queries=20 matched: 9

Myoglobin - Nycticebus coucang (Slow=20 loris)

       P02169        = ;            =         Mass: 17063&n= bsp;   Score: 33     = Expect: 27  Queries=20 matched: 8

Myoglobin - Lepilemur mustelinus (Weasel=20 lemur)

       P02165        = ;            =         Mass: 17088&n= bsp;   Score: 32     = Expect: 29  Queries=20 matched: 8

Myoglobin - Tupaia glis (Tree=20 shrew)

`2.`	`P02170 = ; = Mass: 17210&n= bsp; Score: 69 Expect:<= /B> 0.0063 Queries=20 matched: 11`
	`Myoglobin - Oryctolagus cuniculus=20 (Rabbit)`

`3.`	`Q62010 = ; = Mass: 78986&n= bsp; Score: 53 = Expect: 0.23 Queries=20 matched: 11`
	`Oviduct-specific glycoprotein precursor (Oviductal=20 glycoprotein) (Oviductin) (Estrogen-dependent = ov`

`4.`	`Q9QY78 = ; = Mass: 87951&n= bsp; Score: 43 = Expect: 2.3 Queries=20 matched: 10`
	`Inhibitor of nuclear factor kappa-B kinase subunit = beta (EC=20 2.7.11.10) (I-kappa-B-kinase beta) = (IkB`

`5.`	`P11343 = ; = Mass: 17157&n= bsp; Score: 43 = Expect: 2.8 Queries=20 matched: 9`
	`Myoglobin - Lutra lutra (European river=20 otter)`

`6.`	`P02150 = ; = Mass: 17165&n= bsp; Score: 41 = Expect: 4.4 Queries=20 matched: 9`
	`Myoglobin - Macaca fascicularis (Crab eating macaque) = (Cynomolgus monkey)`

`7.`	`P21692 = ; = Mass: 53918&n= bsp; Score: 39 = Expect: 6.5 Queries=20 matched: 9`
	`Interstitial collagenase precursor (EC 3.4.24.7) = (Matrix=20 metalloproteinase-1) (MMP-1) [Contains: = 18`

`8.`	`P17105 = ; = Mass: 51295&n= bsp; Score: 39 = Expect: 6.6 Queries=20 matched: 7`
	`Inositol-trisphosphate 3-kinase A (EC 2.7.1.127) = (Inositol=20 1,4,5-trisphosphate 3-kinase A) (IP3K = A)`

`9.`	`P63113 = ; = Mass: 17326&n= bsp; Score: 39 = Expect: 6.6 Queries=20 matched: 9`
	`Myoglobin - Canis familiaris = (Dog)`

	`P02159 = ; = Mass: 17353&n= bsp; Score: 39 = Expect: 6.6 Queries=20 matched: 9`
	`Myoglobin - Lycaon pictus (African wild dog) (Cape = hunting=20 dog)`

	`P63114 = ; = Mass: 17326&n= bsp; Score: 39 = Expect: 6.6 Queries=20 matched: 9`
	`Myoglobin - Otocyon megalotis (Bat-eared=20 fox)`

	`P02160 = ; = Mass: 17326&n= bsp; Score: 39 = Expect: 6.6 Queries=20 matched: 9`
	`Myoglobin - Vulpes chama (Cape=20 fox)`

	`P32428 = ; = Mass: 17257&n= bsp; Score: 32 = Expect: 33 Queries=20 matched: 8`
	`Myoglobin - Ondatra zibethicus=20 (Muskrat)`

`10.`	`Q96GQ7 = ; = Mass: 90292&n= bsp; Score: 38 = Expect: 7.6 Queries=20 matched: 10`
	`Probable ATP-dependent RNA helicase DDX27 (EC = 3.6.1.-)=20 (DEAD box protein 27) - Homo sapiens = (Human)`

`11.`	`Q6UWY5 = ; = Mass: 46348&n= bsp; Score: 38 = Expect: 7.8 Queries=20 matched: 10`
	`Olfactomedin-like protein 1 precursor - Homo sapiens=20 (Human)`

`12.`	`Q6ZPK0 = ; = Mass: 73160&n= bsp; Score: 38 = Expect: 7.8 Queries=20 matched: 9`
	`PHD finger protein 21A (BRAF35-HDAC complex protein = BHC80)=20 (mBHC80) (BHC80a) - Mus musculus = (Mouse)`

`13.`	`Q04695 = ; = Mass: 48361&n= bsp; Score: 37 = Expect: 10 Queries=20 matched: 8`
	`Keratin, type I cytoskeletal 17 (Cytokeratin-17) = (CK-17)=20 (Keratin-17) (K17) (39.1) - Homo sapiens = (`

`14.`	`Q9HB96 = ; = Mass: 59415&n= bsp; Score: 37 = Expect: 11 Queries=20 matched: 9`
	`Fanconi anemia group E protein (Protein FACE) - Homo=20 sapiens (Human)`

`15.`	`Q9P2R7 = ; = Mass: 50627&n= bsp; Score: 37 = Expect: 11 Queries=20 matched: 10`
	`Succinyl-CoA ligase [ADP-forming] beta-chain, = mitochondrial=20 precursor (EC 6.2.1.5) (Succinyl-CoA = sy`

`16.`	`Q5R8P0 = ; = Mass: 101961&= nbsp; Score: 36 Ex= pect: 12 Queries=20 matched: 9`
	`C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF=20 synthase) [Includes: Methylenetetrahydrofolate = d`

`17.`	`Q70J99 = ; = Mass: 124288&= nbsp; Score: 36 Ex= pect: 12 Queries=20 matched: 8`
	`Unc-13 homolog D (Munc13-4) - Homo sapiens=20 (Human)`

`18.`	`Q9QW07 = ; = Mass: 135552&= nbsp; Score: 36 Ex= pect: 13 Queries=20 matched: 10`
	`1-phosphatidylinositol-4,5-bisphosphate = phosphodiesterase=20 beta 4 (EC 3.1.4.11) (Phosphoinositide = ph`

`19.`	`Q14533 = ; = Mass: 56875&n= bsp; Score: 36 = Expect: 13 Queries=20 matched: 10`
	`Keratin type II cuticular Hb1 (Type II hair keratin = Hb1)=20 (ghHKb1) (ghHb1) (Keratin-81) (K81) = (K2.9)`

	`O43790 = ; = Mass: 55120&n= bsp; Score: 34 = Expect: 19 Queries=20 matched: 10`
	`Keratin type II cuticular Hb6 (Type II hair keratin = Hb6)=20 (Keratin-86) (K86) (K2.11) - Homo sapiens =`

`20.`	`P14396 = ; = Mass: 17140&n= bsp; Score: 36 = Expect: 13 Queries=20 matched: 8`
	`Myoglobin - Castor fiber (Eurasian=20 beaver)`

Search Parameters

Type of search         : Peptide Mass =
Fingerprint
Enzyme                 : Trypsin
Fixed modifications    : Carbamidomethyl (C)
Variable modifications : Oxidation (M)
Mass values            : Monoisotopic
Protein Mass           : 18 kDa
Peptide Mass Tolerance : =B1 1 Da
Peptide Charge State   : 1+
Max Missed Cleavages   : 2
Number of queries      : 41

Mascot: http://www.matrixscience.com/ =

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var browser_MZ7 =3D 0; var browser_NS3 =3D 0; var browser_NS4 =3D 0; var browser_NS6 =3D 0;=20 var browser_NS7 =3D 0;=20 var browser_OP5 =3D 0;=20 var browser_OP6 =3D 0; var browser_OP7 =3D 0; var browser_KDE =3D 0; var browser_SAF =3D 0; var agt=3Dnavigator.userAgent.toLowerCase(); if(typeof navigator.vendor !=3D "undefined" && navigator.vendor =3D=3D = "KDE"){ var thisKDE =3D agt; var splitKDE =3D thisKDE.split("konqueror/"); var aKDE =3D splitKDE[1] . split("; "); var KDEn =3D parseFloat(aKDE[0]); if (KDEn >=3D 2.2) { browser_KDE =3D 1; // browser_NS6 =3D 1; browser_EXCLUDE =3D 0; } } else if(agt.indexOf('webtv') !=3D -1){browser_EXCLUDE =3D 1;} =20 else if(typeof window.opera !=3D "undefined"){ browser_EXCLUDE =3D 0; if(agt.indexOf("opera/5") !=3D -1 || agt.indexOf("opera 5") !=3D = -1){browser_OP5 =3D 1;} if(agt.indexOf("opera/6") !=3D -1 || agt.indexOf("opera 6") !=3D = -1){browser_OP6 =3D 1;} if(agt.indexOf("opera/7") !=3D -1 || agt.indexOf("opera 7") !=3D = -1){browser_OP7 =3D 1;} } =20 else if(typeof document.all !=3D "undefined"){ browser_EXCLUDE =3D 0; browser_IE =3D 1; if(typeof document.getElementById !=3D "undefined"){ browser_IE5 =3D 1; if(agt.indexOf("msie 6") !=3D -1){ browser_IE6 =3D 1; } } else{browser_IE4 =3D 1;} } =20 else if(typeof document.getElementById !=3D "undefined"){ browser_EXCLUDE =3D 0; if(agt.indexOf("netscape/6") !=3D -1 || agt.indexOf("netscape6") !=3D = -1){browser_NS6 =3D 1;} else if(agt.indexOf("netscape/7") !=3D -1 || agt.indexOf("netscape7") = !=3D -1){browser_NS6 =3D 1; browser_NS7 =3D 1;} else if(agt.indexOf("gecko") !=3D -1){browser_NS6 =3D 1; browser_MZ7 = =3D 1;} if(agt.indexOf("safari") !=3D -1 || (typeof document.childNodes !=3D = "undefined" && typeof document.all =3D=3D "undefined" && typeof = navigator.taintEnabled =3D=3D "undefined")){browser_MZ7 =3D 0; = browser_NS6 =3D 1; browser_SAF =3D 1;} } =20 else if((agt.indexOf('mozilla') !=3D -1) && = (parseInt(navigator.appVersion) >=3D 3)){ browser_EXCLUDE =3D 0; if (parseInt(navigator.appVersion) >=3D 4) { browser_NS4 =3D 1; } else { browser_NS3 =3D 1; } if(typeof navigator.mimeTypes['*'] =3D=3D "undefined"){ browser_EXCLUDE =3D 1; browser_NS4 =3D 0; browser_NS3 =3D 0; } } =20 if(agt.indexOf('escape') !=3D -1){browser_EXCLUDE =3D 1; browser_NS3 = =3D 0; browser_NS4 =3D 0;} =20 if(typeof navigator.__ice_version !=3D "undefined"){browser_EXCLUDE = =3D 1; browser_IE4 =3D 0;} var sTemp =3D "Browser detected as "; if (browser_EXCLUDE) { sTemp +=3D "EXCLUDE " } if (browser_IE) { sTemp +=3D "IE " } if (browser_IE4) { sTemp +=3D "IE4 " } if (browser_IE5) { sTemp +=3D "IE5 " } if (browser_IE6) { sTemp +=3D "IE6 " } if (browser_MZ7) { sTemp +=3D "MZ7 " } if (browser_NS3) { sTemp +=3D "NS3 " } if (browser_NS4) { sTemp +=3D "NS4 " } if (browser_NS6) { sTemp +=3D "NS6 " } if (browser_NS7) { sTemp +=3D "NS7 " } if (browser_OP5) { sTemp +=3D "OP5 " } if (browser_OP6) { sTemp +=3D "OP6 " } if (browser_OP7) { sTemp +=3D "OP7 " } if (browser_KDE) { sTemp +=3D "KDE " } if (browser_SAF) { sTemp +=3D "SAF " } // alert(sTemp); ------=_NextPart_000_001B_01C87EC3.15349830--

`1.`	`P68083 = ; = Mass: 17072&n= bsp; Score: 163 Expect:&n= bsp;2.5e-012 Queries=20 matched: 20`
	`Myoglobin - Equus burchelli (Plains zebra) (Equus=20 quagga)`

	`P68082 = ; = Mass: 17072&n= bsp; Score: 163 Expect:&n= bsp;2.5e-012 Queries=20 matched: 20`
	`Myoglobin - Equus caballus = (Horse)`

	`P02166 = ; = Mass: 17052&n= bsp; Score: 76 Expect:<= /B> 0.0014 Queries=20 matched: 12`
	`Myoglobin - Perodicticus potto edwarsi=20 (Potto)`

	`P02168 = ; = Mass: 17091&n= bsp; Score: 54 = Expect: 0.2 Queries=20 matched: 10`
	`Myoglobin - Galago crassicaudatus (Thick-tailed = galago)=20 (Otolemur crassicaudatus)`

	`P02186 = ; = Mass: 17116&n= bsp; Score: 47 = Expect: 0.9 Queries=20 matched: 10`
	`Myoglobin - Elephas maximus (Indian=20 elephant)`

	`P02187 = ; = Mass: 17142&n= bsp; Score: 47 = Expect: 0.9 Queries=20 matched: 10`
	`Myoglobin - Loxodonta africana (African=20 elephant)`

	`P02167 = ; = Mass: 16944&n= bsp; Score: 43 = Expect: 2.4 Queries=20 matched: 9`
	`Myoglobin - Nycticebus coucang (Slow=20 loris)`

	`P02169 = ; = Mass: 17063&n= bsp; Score: 33 = Expect: 27 Queries=20 matched: 8`
	`Myoglobin - Lepilemur mustelinus (Weasel=20 lemur)`

	`P02165 = ; = Mass: 17088&n= bsp; Score: 32 = Expect: 29 Queries=20 matched: 8`
	`Myoglobin - Tupaia glis (Tree=20 shrew)`

		Help
	Significance threshold p<	Max. number of hits

= Mascot Search=20 Results

Probability Based Mowse Score

Concise Protein Summary Report

Search Parameters