From: Subject: Concise Summary Report (PRL-S5) Date: Wed, 5 Mar 2008 13:34:29 -0500 MIME-Version: 1.0 Content-Type: multipart/related; type="text/html"; boundary="----=_NextPart_000_0024_01C87EC5.A3A7CF40" X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 This is a multi-part message in MIME format. ------=_NextPart_000_0024_01C87EC5.A3A7CF40 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Content-Location: http://localhost/mascot/cgi/master_results.pl?file=../data/20080305/F002843.dat Concise Summary Report (PRL-S5)

= Mascot Search=20 Results

User    =
        : Protea PRL
Email           : 
Search title    : PRL-S5
Database        : SwissProt 20050315 (176469 sequences; 63878124 =
residues)
Taxonomy        : Mammalia (mammals) (34391 sequences)
Timestamp       : 5 Mar 2008 at 18:33:39 GMT
Warning         : Specifying a protein mass disables mixture mode in =
PMF-search
Top Score       : 71 for K1CS_MOUSE, =
(P19001) Keratin, type I cytoskeletal 19 (Cytokeratin 19) (K19) (CK =
19)

Probability Based Mowse Score

Ions score is -10*Log(P), where P = is the=20 probability that the observed match is a random event.
Protein scores = greater=20 than 58 are significant (p<0.05).

3D"Score=20

=20

Concise Protein Summary Report

Help
  Significance threshold p< Max. number of hits
= =20 =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 =20 = =20 =20 = =20 =20 = =20 =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 = =20 =20 =20 =20 = = = = =20

  =20

1.     K1CS_MOUSE       &= nbsp;           &n= bsp;    Mass: 44515   &nbs= p;Score: 71     Expect:<= /B> 0.0028  Queries=20 matched: 13
  (P19001) Keratin, type I cytoskeletal 19 (Cytokeratin = 19)=20 (K19) (CK 19)

2.     SKI_MOUSE       &n= bsp;           &nb= sp;     Mass: 37845   = ; Score: 63     Expect:<= /B> 0.019  Queries=20 matched: 11
  (Q60698) Ski oncogene (C-ski)=20 (Fragment)
3.     RORA_MOUSE       &= nbsp;           &n= bsp;    Mass: 58807   &nbs= p;Score: 63     Expect:<= /B> 0.019  Queries=20 matched: 11
  (P51448) Nuclear receptor ROR-alpha (Nuclear receptor = RZR-alpha)
4.     T23O_RAT       &nb= sp;           &nbs= p;      Mass: 47826  =   Score: 61     Expect:<= /B> 0.03  Queries=20 matched: 13
  (P21643) Tryptophan 2,3-dioxygenase (EC 1.13.11.11)=20 (Tryptophan pyrrolase) (Tryptophanase) = (Tryptop
5.     DIAP1_MOUSE       =             &= nbsp;   Mass: 139255   Score= : 57     Expect: 0.072 = ; Queries=20 matched: 11
  (O08808) Diaphanous protein homolog 1 = (Diaphanous-related=20 formin 1) (DRF1) (mDIA1) (p140mDIA)
6.     SCEL_MOUSE       &= nbsp;           &n= bsp;    Mass: 73128   &nbs= p;Score: 57     Expect: = 0.075  Queries=20 matched: 13
  (Q9EQG3) Sciellin
7.     CYR61_HUMAN       =             &= nbsp;   Mass: 41998    = Score: 56     Expect: 0.084=   Queries=20 matched: 12
  (O00622) CYR61 protein precursor (Cysteine-rich, = angiogenic=20 inducer, 61) (Insulin-like growth = facto
8.     NR1D2_RAT       &n= bsp;           &nb= sp;     Mass: 64150   = ; Score: 56     Expect:&= nbsp;0.086  Queries=20 matched: 10
  (Q63504) Orphan nuclear receptor NR1D2 (Rev-erb-beta) = (EAR4)
9.     ZN222_HUMAN       =             &= nbsp;   Mass: 52498    = Score: 55     Expect: 0.11&= nbsp; Queries=20 matched: 13
  (Q9UK12) Zinc finger protein = 222
10.    P85B_MOUSE       &= nbsp;           &n= bsp;    Mass: 81201   &nbs= p;Score: 53     Expect: = 0.17  Queries=20 matched: 13
  (O08908) Phosphatidylinositol 3-kinase regulatory = beta=20 subunit (PI3-kinase p85-beta subunit) = (PtdIn
11.    PAIRB_RAT       &n= bsp;           &nb= sp;     Mass: 44727   = ; Score: 53     Expect:&= nbsp;0.18  Queries=20 matched: 13
  (Q6AXS5) Plasminogen activator inhibitor 1 = RNA-binding=20 protein (PAI1 RNA-binding protein 1) = (PAI-RB
12.    LSM11_MOUSE       =             &= nbsp;   Mass: 39883    = Score: 53     Expect: 0.18&= nbsp; Queries=20 matched: 14
  (Q8BUV6) U7 snRNA-associated Sm-like protein=20 LSm11
13.    SKI_HUMAN       &n= bsp;           &nb= sp;     Mass: 79955   = ; Score: 53     Expect:&= nbsp;0.18  Queries=20 matched: 11
  (P12755) Ski oncogene = (C-ski)
14.    TBBX_HUMAN       &= nbsp;           &n= bsp;    Mass: 49799   &nbs= p;Score: 52     Expect: = 0.22  Queries=20 matched: 10
  (P68371) Tubulin beta-? chain (Tubulin beta-2=20 chain)
       TBBX_MOUSE       &= nbsp;           &n= bsp;    Mass: 49799   &nbs= p;Score: 52     Expect: = 0.22  Queries=20 matched: 10
  (P68372) Tubulin beta-? = chain
       TBB_PIG       &nbs= p;            = ;       Mass: 49829 &= nbsp;  Score: 50     Ex= pect: 0.34  Queries=20 matched: 10
  (P02554) Tubulin beta = chain
15.    LAMA1_HUMAN       =             &= nbsp;   Mass: 336941   Score= : 51     Expect: 0.26 =  Queries=20 matched: 10
  (P25391) Laminin alpha-1 chain precursor (Laminin A=20 chain)
16.    ARHGB_HUMAN       =             &= nbsp;   Mass: 167601   Score= : 51     Expect: 0.29 =  Queries=20 matched: 9
  (O15085) Rho guanine nucleotide exchange factor 11=20 (PDZ-RhoGEF)
17.    TYPH_MOUSE       &= nbsp;           &n= bsp;    Mass: 49305   &nbs= p;Score: 50     Expect: = 0.33  Queries=20 matched: 9
  (Q99N42) Thymidine phosphorylase (EC 2.4.2.4) = (TdRPase)=20 (TP)
18.    EDN1_FELCA       &= nbsp;           &n= bsp;    Mass: 22899   &nbs= p;Score: 50     Expect: = 0.33  Queries=20 matched: 14
  (Q5NRQ1) Endothelin-1 precursor (ET-1) = (Preproendothelin-1)=20 (PPET1)
19.    ZN227_HUMAN       =             &= nbsp;   Mass: 91974    = Score: 50     Expect: 0.35&= nbsp; Queries=20 matched: 12
  (Q86WZ6) Zinc finger protein = 227
20.    ACINU_HUMAN       =             &= nbsp;   Mass: 151797   Score= : 50     Expect: 0.38 =  Queries=20 matched: 10
  (Q9UKV3) Apoptotic chromatin condensation inducer in = the=20 nucleus (Acinus)

Search Parameters

Type of search         : Peptide Mass =
Fingerprint
Enzyme                 : Trypsin
Mass values            : Monoisotopic
Protein Mass           : 14 kDa
Peptide Mass Tolerance : =B1 0.2 Da
Peptide Charge State   : 1+
Max Missed Cleavages   : 2
Number of queries      : 202

Mascot:  http://www.matrixscience.com/ =

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agt.indexOf("netscape7") = !=3D -1){browser_NS6 =3D 1; browser_NS7 =3D 1;} else if(agt.indexOf("gecko") !=3D -1){browser_NS6 =3D 1; browser_MZ7 = =3D 1;} if(agt.indexOf("safari") !=3D -1 || (typeof document.childNodes !=3D = "undefined" && typeof document.all =3D=3D "undefined" && typeof = navigator.taintEnabled =3D=3D "undefined")){browser_MZ7 =3D 0; = browser_NS6 =3D 1; browser_SAF =3D 1;} } =20 else if((agt.indexOf('mozilla') !=3D -1) && = (parseInt(navigator.appVersion) >=3D 3)){ browser_EXCLUDE =3D 0; if (parseInt(navigator.appVersion) >=3D 4) { browser_NS4 =3D 1; } else { browser_NS3 =3D 1; } if(typeof navigator.mimeTypes['*'] =3D=3D "undefined"){ browser_EXCLUDE =3D 1; browser_NS4 =3D 0; browser_NS3 =3D 0; } } =20 if(agt.indexOf('escape') !=3D -1){browser_EXCLUDE =3D 1; browser_NS3 = =3D 0; browser_NS4 =3D 0;} =20 if(typeof navigator.__ice_version !=3D "undefined"){browser_EXCLUDE = =3D 1; browser_IE4 =3D 0;} var sTemp =3D "Browser detected as "; if (browser_EXCLUDE) { sTemp +=3D "EXCLUDE " } if (browser_IE) { sTemp +=3D "IE " } if (browser_IE4) { sTemp +=3D "IE4 " } if (browser_IE5) { sTemp +=3D "IE5 " } if (browser_IE6) { sTemp +=3D "IE6 " } if (browser_MZ7) { sTemp +=3D "MZ7 " } if (browser_NS3) { sTemp +=3D "NS3 " } if (browser_NS4) { sTemp +=3D "NS4 " } if (browser_NS6) { sTemp +=3D "NS6 " } if (browser_NS7) { sTemp +=3D "NS7 " } if (browser_OP5) { sTemp +=3D "OP5 " } if (browser_OP6) { sTemp +=3D "OP6 " } if (browser_OP7) { sTemp +=3D "OP7 " } if (browser_KDE) { sTemp +=3D "KDE " } if (browser_SAF) { sTemp +=3D "SAF " } // alert(sTemp); ------=_NextPart_000_0024_01C87EC5.A3A7CF40--